An efficiency comparison of different in vitro fertilization methods: IVF, ICSI, and PICSI for embryo development to the blastocyst stage from vitrified porcine immature oocytes | Porcine Health Manag To perform insemination, the semen sample was obtained from one Landrace boar, using the gloved hand method at a commercial insemination center, diluted in Duragen (Magapor, México) 1:2 (v:v), then transported to the laboratory at 16 °C within 2 h after collection. In oocytes, viability significantly decreased after IVM in vitrified oocytes. but only a few of them have evaluated which fertilization procedure enhances blastocyst rates in vitrified oocytes.